How to Interpret Your Environmental Test Results

Environmental laboratory reports can be difficult to understand. This guide provides general information about common air, surface, and dust-testing results and how they may be interpreted.

Laboratory results are an important part of understanding an indoor environment, but they should not be considered alone. Moisture conditions, building history, HVAC systems, contaminated dust, sampling locations, occupant concerns, and environmental assessment findings may all affect the overall interpretation.

Mold Surface and Air Testing

Air and surface sampling can help identify the types and concentrations of mold captured in the areas tested. Results are interpreted by considering the mold species identified, total concentrations, indoor-to-outdoor comparisons, sampling locations, marker molds, hyphal fragments, and evidence of current or previous moisture.

It is important to understand that this testing provides a limited snapshot—not a complete picture of everything present within a home or building. An air sample collects a relatively small volume of air from one location during a brief period. Surface testing represents only the specific area sampled. Contamination may be present elsewhere without being captured in that particular sample.

Results can also vary based on humidity, airflow, HVAC operation, disturbance, occupancy, weather, and other conditions at the time of testing. Concealed contamination within walls, ceilings, flooring systems, insulation, subflooring, crawlspaces, attics, or HVAC components may not be reflected in the laboratory results.

For these reasons, air and surface testing are most useful when evaluated alongside a comprehensive environmental assessment, moisture readings, building history, occupant observations, and other available evidence.

How to Read Your Results

Low – Considered safe for re-entry. Minimal concern for sensitized individuals.

Medium – May provoke mild symptoms in highly sensitive patients. Monitor closely.

High/Abnormal/Elevated – Unsafe for CIRS patients. Urgent remediation required before safe occupancy.

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Quick Interpretation Guide

HERTSMI-2 → Measures 5 toxigenic mold species. Scores >15 are unsafe for CIRS patients (Dr. Shoemaker standard).

Endotoxin → Indicates bacterial fragments (from dampness or HVAC issues). High levels can worsen respiratory, neurological, and immune symptoms.

Actinobacteria → Soil-based bacteria common in water-damaged buildings. High dominance & prevalence suggest a contaminated indoor microbiome.

Beta Glucans → are structural components of the cell walls of most fungi, including molds. So, when lab tests detect elevated beta glucan levels in air, dust, or surfaces, it indicates fungal biomass is present — whether the mold is alive or dead.

Testing for CIRS and Other Sensitized Individuals

Some occupants may require more detailed environmental testing and more conservative interpretation criteria. Depending on the individual and the concerns identified, this may include ERMI, HERTSMI-2, Actinobacteria, endotoxin, beta-glucan, or mycotoxin testing.

How to Read Your Results

Desired – Considered safe for re-entry. Minimal concern for sensitized individuals.

Acceptable – May provoke mild symptoms in highly sensitive patients. Monitor closely.

Unacceptable – Unsafe for CIRS patients. Urgent remediation required before safe occupancy.

Learn more about hyphal fragments here. Learn more about indicator molds here

What Should You Do if Levels Are Elevated?

 

Borderline and lower:

  • Increase cleaning and monitoring. Retest in 3–6 months.

Elevated or Very High:

  • Consult a CIRS-aware Indoor Environmental Professional (IEP)
  • Identify & eliminate moisture sources
  • Remove contaminated materials
  • Perform deep cleaning & decontamination
  • Retest before re-occupying

References

Dr. Ritchie Shoemaker – Surviving Mold Protocol
Thorne, P.S. et al. (2009). Indoor Endotoxin Exposure and Respiratory Health
Pestka, J.J. (2008). Actinomycetes & Environmental Health
EPA & ISEAI guidance on indoor microbial safety

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